2006. Medical Applications:-In medicine, bacteria play a vital role for the synthesis … This very low efficiency, combined with safety concerns, presents a serious obstacle to the application of reproductive cloning. The present chapter reviews recent advances that have occurred in therapeutic cloning … This chapter explores some of the immediate uses of cloning technology in medicine and agriculture, and focuses on the applications … Show details Methods Mol Biol Actions. For instance, Dolly was the only clone to be born live out of a total of 277 cloned embryos. Methods Mol Biol. Cloning technology has made several steps forward in recent years, most notably in somatic cell cloning and in combining cloning with transgenic technologies. Therapeutic cloning, where the nucleus from a donor cell is transferred into an enucleated oocyte in order to extract pluripotent embryonic stem cells, offers a potentially limitless source of cells for tissue engineering applications. Future and Applications of Cloning Alan O Trounson. Search in PubMed Search in NLM Catalog Add to Search . Application of Gene Cloning:-Gene cloning is applicable in many fields of science such as medicine and agriculture. 2006;348:319-32. doi: … Reproductive cloning is a very inefficient technique and most cloned animal embryos cannot develop into healthy individuals.