Germination testing in sand of maize landraces accessions from gene bank gavebetter results with higher correlation with field emergence than filter paper method (Babi. Use of different substrate may lead to discrepancy between testing results. The primary aim of ISTA Rules is to provide testing methods for seeds designated for growing of crops or production of the plants. There is no universally accepted vigour test for all kinds of seeds. Tests are available to address nearly all phytosanitary and quality assurance concerns. Ideally, make three dilutions of seed such that the smallest quantity gives at least 2.0 mg/L DO depletion and the largest quantity results in at least 1.0 mg/L DO residual after 5 d of incubation. Seed Health Testing The Iowa State University Seed Health Testing Laboratory tests for more than 350 viral, bacterial, and fungal pathogens on most crops, including corn, soybeans, vegetables, and flowers using a variety of methods. Purity tests of most commercial seed lots of Eucalyptus are not made because it is difficult or impossible to separate the seed from the chaff of some species (Boland et al. Chapter 9 SEED TESTING (Contd.) G. R. Orton, of the West Virginia Agricultural Experiment Station, in 1931 estimated losses in the United States caused by seedborne diseases to amount to 2.3 percent of the total crop of wheat, 3.1 percent of the barley crop, 3.2 percent of the oats crop, and 6.r percent of the bean crop. Species in which seed and chaff are extremely similar in size, weight and colour include E. cloeziana, E. regnans and E. delegatensis. Right choice of seed testing method is crucial in order to obtain adequate estimation of seed lot quality. 1)1e determination of following vigour tests will be useful in gaining additional infor- mation on seed quality. Combining purity and Germination Tests. 1980). international methods for testing the most serious seed-transmitted organ- isms are needed. performance of seed vigour test methods for different crops: (i) Reproducibility ofvigour method 242 (ii) The relationship between vigour test results and seedling emer-gence in field soil. Determine the DO uptake per milliliter of seed added to each bottle using either the slope method or the ratio method. Direct and indirect tests . 1. Seed health testing based on these principles will help to ensure that healthy seed is available to growers and prevent the introduction and spread of pathogenic organisms. This is the seed control. The need for seed testing methods that are reliable and reproducible among its accredited member laboratories is therefore a basic need for ISTA. PDF | Seed is the product of fertilized ovule that consists of embryo, seed coat, and cotyledon (s). AMENDMENTS TO TEST METHODS .