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Sometimes turbidity may be due to phosphate or carbonate precipitation. Lindeman's test for diacetic acid. it is so then glacial acetic acid clear up the turbidity .if it is due to protein then precipitation will be there after the addition of acetic acid 4. 2. Veterinary Laboratory, Ministry of Agriculture Weybridge REFERENCES (1) A.C.H. Rothera's test. Han’s method for betahydroxybutyric acid. Place 5 to 10 ml of clear urine in test tube; Boil the upper portion over a flame. Updates Video Tutorials Practical Videos (MBBS+BDS+DMLT) Rothera's Test || Rothera's Test for Ketone Bodies || Biochemistry Practical Video Tutorials Practical Videos (MBBS+BDS+DMLT) Rothera's Test || Rothera's Test for Ketone Bodies || Biochemistry Practical Gerhard's test for diacetic acid. It is done by taking 5 mL of urine and adding ten drops of concentrated ammonia water and solid ammonium sulfate. 3. If turbidity develops add 1-2 drops of glacial acetic acid. Principle. Rothera's test: a test for the presence of ketone bodies, diacetic acid and acetone in urine. Rothera's test (roth-er-ăz) n. a method of testing urine for the presence of acetone or acetoacetic acid – a sign of diabetes mellitus. rothera's test procedure, Principle, Uses, reagents Preparation and results Interpretation with normal reference range - test for ketone bodies. Rothera, J. Physiol xxxii: (1908) 491-(2) Harding, Ruttan, Biochem. ROTHERA'S TEST ON MILK The Diagnostic Value of Rothera's Test on Milk A.B. Quick Reference (roth-er-ăz) a method of testing urine for the presence of acetone or acetoacetic acid – a sign of diabetes mellitus. Rothera's test for acetone. May also be applied to milk. Paterson, Veterinary Laboratory, Ministry of Agriculture, Weybridge. Principle of Rothera’s Test Acetoacetic acid and acetone react with alkaline solution of sodium nitroprusside to form a purple colored complex. 5. Tablet test. [ A. C. H. Rothera (1880–1915), Australian biochemist] Source for information on Rothera's test: A Dictionary of Nursing dictionary. The Rothera nitroprusside test looks for ketone bodies in urine.

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